Bright CD22 Expression Using ASR Reagent

February 01, 2019

BlueskyReddit
Toxicology
University of Kentucky
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

CD22 PE

Catalog Number:

340708

Browse Similar Products: CD22 Flow Cytometry Antibodies

Identifying CD22 on B cells is an important part of our research. It inhibits B cell receptor signaling via alpha 2,6 sialic acid ligand binding activity, and is used as a marker for certain therapies in leukemia. Epratuzumab and other anti-CD22 antibodies may have therapeutic value as part of radioimmunotherapy.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMC

Primary Incubation

20 µL of neat antibody for 30 minutes at 4°C

Blocking Agent

None needed

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

In FL-2 (PE channel)

Results Summary

Results using this antibody show excellent, high intensity staining on B cells (confirmed by staining with CD20:PCP) as shown in the figure.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use, straight from the vial

The Bad

N/A

The Bottom Line

Great antibody. Specific, strong staining.

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