Good TCF1/TCF7 Antibody

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signalling Technology

Product Name:

TCF1/TCF7 (C63D9) Rabbit mAb (Alexa Fluor® 647 Conjugate)

Catalog Number:

6709

Our lab studies the function of T cells in an influenza model in mice. One of our interests is to see how TCF1 expression may change due to different treatments we give the mice. This antibody is bright enough that we can see the expression using intracellular staining.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lung cells

Primary Incubation

Cells were stained in 50 µL Perm buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100 µL FACS buffer.

Blocking Agent

FACS buffer

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

This antibody allows us to see TCF1 expression and also how it changes in different cell groups.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Bright and stable

The Bad

NONE

The Bottom Line

This antibody works efficiently to stain mouse TCF1 expression.

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