Good Mouse Ki67 Antibody

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Anti-mouse Ki67 BV605

Catalog Number:

652413

Our lab studies the function of T cells in an influenza model in mice. One of our interests is studying the proliferation of these T cells under different treatments. We chose this antibody because it’s bright and allows us to see any changes in Ki67 expression. The antibody is bright and stable.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lung cells

Primary Incubation

Cells were stained in 50 µL Perm buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100 µL FACS buffer.

Blocking Agent

FACS buffer

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

This antibody allows us to see any differences in Ki67 expression in our T cell treatment groups.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright and stable

The Bad

NONE

The Bottom Line

This antibody works efficiently to stain mouse Ki67 expression.

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