Good Bcl-2 Antibody

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-Bcl-2 Antibody

Catalog Number:

633508

Our lab studies the function of T cells in an influenza model in mice. In some of our models, we are interested in the life cycle of the T cells after different treatments. Since this is an intracellular stain, we wanted to choose something bright. This antibody is bright and stable.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lung cells

Primary Incubation

Cells were stained in 50 µL Perm buffer with antibody at 1:50; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100 µL FACS buffer.

Blocking Agent

FACS buffer

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

This antibody works well with the intracellular staining protocol.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright and stable

The Bad

NONE

The Bottom Line

This antibody is bright and allows us to clearly look at BCL2 expression.

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