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Anti-human CD80 BV421
305222
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Anti-human CD80 BV421 is an excellent bright antibody to measure activation of human peripheral blood DC after pIC stimulation
Flow Cytometry
Human PBMC
1 µL/test, RT, 15 min
Housemade blocking buffer containing normal sera
N/A
LSR Fortessa
Human PBMC were stimulated with pIC overnight and activation on dendritic cells (CD11c+MHC-II+) was measured using anti-CD80 BV421. Blue histogram: unstimulated; orange histogram: pIC. The antibody is bright enough to be used at a 5x lower test size than recommended (1 µL instead of 5 µL) and results in a dynamic signal.
Very bright, dynamic signal
No complaints
Excellent antibody to measure DC activation