Thermo Fisher Scientific
Lysotracker Red DND-99
L7528
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Lysotracker Dye is used to investigate the changes in the amount of lysosome and to observe the fusion between the lysosome and the internalized microbes.
Fluorescence Microscopy
AGS gastric cancer cell line
(1) Add the pre-warmed (37°C) probe-containing medium and incubate the cells for 30 minutes. (2) Replace the loading solution with fresh medium and observe the cells using a fluorescence microscope fitted with the correct filter set.
Ensure the cells are sufficiently stained by carrying out a pretest for the incubation time of the cells in medium containing the dye.
The images obtained are specific with little or no background noise detected.
N/A
Easy to use compared to immunofluorescence.
NIL
Recommended for those who are carrying out studies on the lysosome.