Abcam
Anti-EZH2
ab191250
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EZH2 is an epigenetic regulator that is commonly dysregulated in most cancer types. Here we assess the expression of EZH2 in the PC3 prostate cancer cell line. The antibody works as expected.
Western Blot
PC3 whole-cell lysate
1:1000, Overnight at 4°C
5% skimmed milk - 30 min room temperature
1:10000, anti-rabbit HRP conjugated antibody, 30 min at room temperature
N/A
ECL - film
Antibody worked well and was able to detect the protein of interest when comparing knockdown and control cell lysates. Size ~ 100 kDa.
Works as expected.
Detects EZH2 with no background bands observed