Abcam
Anti-GOLPH3
ab69171
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GOLPH3 is a Golgi membrane protein reported to play a role in regulating Golgi trafficking. Here we assess the expression of GOLPH3 in the PC3 prostate cancer cell line. The antibody works well.
Western Blot
PC3 whole-cell lysate
1:1000, Overnight at 4°C
5% skimmed milk - 30 min room temperature
1:10000, anti-mouse HRP conjugated antibody, 30 min at room temperature
N/A
ECL - film.
Antibody worked and was able to detect the protein of interest when comparing knockdown and control cell lysates. Size ~ 30 kDa.
Detects protein of interest at a reasonable dilution.
Required loading of excess total protein to visualize band.
Can detect GOLPH3 by WB