Santa Cruz Biotechnology
SIRT1 Antibody (33.36)
sc-135792
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I worked with this antibody for mouse tissues and cell line protein lysate. This is a very good antibody giving very clear band at 120 kDa.
Western Blot
Mouse tissue lysate
SIRT1 (1:500 dilution), Overnight at 4°C
Odyssey blocking buffer (1:1)
Goat anti-Mouse IgG, LICOR (1:5000 dilution) at RT for 1 hr
N/A
Scanned on Odyssey® CLx (auto scan 700 & s800).
I have worked with this antibody from Santa Crutz Biotechnology. This is one of the best antibodies to work with. Easy to standardize and gives very clear band at 12 kDa.
Very good
None
I would highly recommend fellow researchers to use this antibody for their western blot.