Not Very Sensitive

UCLA
Pediatrics
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BosterBio

Product Name:

A2M ELISA kit

Catalog Number:

EK1118

Our research focuses on in vitro modeling of organ fibrosis and drug discovery. The protocol for this product was easy to perform. However, there was some amount of variability with the replicates.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Cell culture supernatant

Protocol Overview

With my first experiment, I performed all the incubations at 37 degrees. The second time around, I performed it at room temperature. The overall variability with both incubation temperatures, I observed variability between the replicates. Reproducibility was only reasonable.

Tips

Follow protocol to the T.

Results Summary

Reasonable sensitivity and reproducibility

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use

The Bad

Reproducibility

The Bottom Line

Was effective in detecting A2M levels at pg levels, but reproducibility was only reasonable.

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