Alexa Fluor 700 Anti-Mouse CD3 Antibody

December 20, 2018

Harvard Medical School
Harvard
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 700 anti-mouse CD3 Antibody

Catalog Number:

100216

I am studying molecular anomalies in signaling pathways leading to cancers and the development of anti-cancer immune therapies. This antibody is great for detecting CD3 on mouse immune cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse tissue single-cell suspension

Primary Incubation

1:100 dilution for 1 hour on ice

Blocking Agent

Blocking with 2% BSA and Fc block (1:100)

Secondary Incubation

1:50 dilution on ice with intracellular staining antibodies

Tertiary Incubation

None

Detection

FACS

Results Summary

Efficient CD3 expression detection on mouse immune cells

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good antibody for efficient detection of CD3 on mouse immune cells

The Bad

None

The Bottom Line

The antibody works great with mouse immune cells, requires a minimum of 20 minutes on ice for good staining.

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