Vibratome for Brain Sectioning

January 03, 2019

BlueskyReddit
Centre for Cellular & Molecular Biology
Project JRF
Postdoctoral Researcher

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Performance

Ease-of-Optimization

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Company:

Leica

Product Name:

Leica VT1000 S Vibrating blade microtome

Catalog Number:

14047235613

With this Leica vibratome, it is very easy to take sections of the fixed brain tissue sample. This vibratome uses a vibrating principle to section the brain and can give quality sections up to 5 micron thickness. Very easy to use and clean up, unlike other sectioning equipment (Cryostat and Microtome used for paraffin blocks). You just require 1xPBS, superglue, and a razor blade.

Experimental Design and Results Summary

Tips

Always be careful when you move your hand over the razor blade while fixing the blade and cleaning. Clean and maintain well after every use for long-lasting performance.

DOI or PMID #

N/A

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Summary

The Good

Very quick and easy for sectioning brain tissue unlike Microtome and Cryostat.

The Bad

Sometime it is difficult to pick sections and mount in a slide if the sections are so thin (<5 micron).

The Bottom Line

Highly recommended for sectioning brain tissue.

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