Convenient Pre-Cast Gels for Western Blot, 17-well

University of Toronto
LMP
Graduate Student

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Company:

Thermo Fisher Scientific

Product Name:

BOLT 4-12% Bis-Tris Plus 17-well

Catalog Number:

NW04127BOX

Western blots have been one of the most common techniques for quantifying protein expression in a biological sample. Although most WB procedures take 1–2 days to perform, nowadays companies provide reagents that can speed up the process and provide more consistent results. Here, I utilized the BOLT 4-12% Bis-Tris Plus 17-well pre-cast gels for running a WB. The gels are excellent and can run up to 16 samples.

Experimental Design and Results Summary

Application

WB

Starting Material

A375 whole-cell lysate

Protocol Overview

These gels come 10 in a box and each gel contains 17 wells. This gel is utilized with the BOLT running apparatus and buffer system.

Tips

Remove the comb and white tape at the bottom of the gel BEFORE running.

Results Summary

The gel can be run at high voltages that can minimize running times. Resolution of bands was good!

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Pre-cast gel with 17 wells!!

The Bad

Need to run these gels using the BOLT running buffer and WB system

The Bottom Line

Excellent pre-cast gels for running up to 16 samples.

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