Clean METTL3 Western Blot Antibody

December 17, 2018

BlueskyReddit
LMP
University of Toronto
Graduate Student

Overall

Quality of Results

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Company:

Cell Signaling Technology

Product Name:

Anti-METTL3

Catalog Number:

96391

Recent studies have demonstrated the impact of RNA modifications in cellular expression of genes. Here, I assess the expression of METTL3, which is a ‘bona fide’ writer of the RNA modification N-6 methyladenoisne (m6A). The antibody is clean and works as expected.

Experimental Design and Results Summary

Applications

Western Blot

Sample

A375 whole-cell lysate

Primary Incubation

1:1000, Overnight at 4°C

Blocking Agent

5% skimmed milk - 10 min room temperature

Secondary Incubation

1:10000, anti-rabbit HRP conjugated antibody, 30 min at room temperature

Tertiary Incubation

N/A

Detection

ECL reagent – film

Results Summary

Readily detects METTL3 with limited background. Approx. size 65–70 kDa.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Clean antibody that works as expected.

The Bad

N/A

The Bottom Line

Great antibody to probe for METTL3 in WB.

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