MilliporeSigma
Acetate Colorimetric Assay Kit
MAK086
Info: Browse Acetate Colorimetric Assay Kits
This kit from Sigma is sufficient to measure a half 96-well plate of samples, even though in their description it can measure 1 plate of samples, because you need half of the wells for background controls!
Assay measurement
N/A
Tissue (10 mg) or cells (1 × 106) can be homogenized in 100 μL of ice-cold Acetate Assay Buffer. Centrifuge the samples at 13,000 × g for 10 minutes to remove insoluble material. Serum and other liquid samples can be directly added to wells. Bring samples to a final volume of 50 μL with Acetate Assay Buffer. Set up the Reaction Mixes according to the scheme in Table provided.
Only works with less than 40 samples at a time!
The assay kit itself works great at detecting acetate in samples, in my case the supernatant of cells.
Accurate
Only measures less than half the number of samples as described
Works