Specific Calpain I Antibody

Molecular Microbiology
Washington University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Rabbit polyclonal to calpain 1

Catalog Number:

ab28257

I am usually very satisfied with Abcam antibodies, and it's the same this time. This anti-calpain I antibody works great against mouse tissue in paraffin-embedded sections. The signal is strong and clear.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse tissue in paraffin

Primary Incubation

1:5000

Blocking Agent

Goat serum

Secondary Incubation

1:1000

Tertiary Incubation

None

Detection

IHC kit from Abcam

Results Summary

This antibody recognize the mono terminal end of the large subunit of calpain 1, and can be used to discriminate between latent and amino-acid processed forms. It reacts with mouse, rat, and human, and can be used in WB and ICC/IF. It does not cross react with the other calpain family members. We can clearly observe the high level of calpain I in the tissues, and specifically localized to certain place. See brown color in image.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific, high dilution

The Bad

None

The Bottom Line

Great antibody against calpain I

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