CD45 Monoclonal Antibody (2D1), eFluor 450, from eBioscience

Innate Immunity
Deutsches Rheuma-Forschungszentrum
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

CD45 Monoclonal Antibody (2D1), eFluor 450, eBioscience™

Catalog Number:

48-9459-41

Antibody against human CD45 in fluorochrome eF450 was used in a panel to identify human lymphocyte populations in peripheral blood. Staining was clear and bimodal as detected through flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

15 min, 37ºc

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII Fortessa

Results Summary

Staining is clear in human PBMCs. Although staining is bimodal, the positive population is less bright and more of a shift than other CD45 antibodies in the market. Nevertheless, it is a good, reliable, clear staining.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Clear, bimodal staining, reliable

The Bad

Less bright than other options

The Bottom Line

Good, clear, reliable antibody to be used as a lymphocyte marker in flow cytometry panels.

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