Great Pax-5 Antibody

December 14, 2018

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

Alexa Fluor® 647 anti-Pax-5 Antibody

Catalog Number:

649704

We are interested in studying the mechanism of T cell differentiation through modulating the mTOR pathway. This Pax-5 transcription factor antibody was used to distinguish B cells. It's easy to use and has great specificity.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

Permeabilization buffer

Blocking Agent

None

Secondary Incubation

Stain with Pax5-AF647

Tertiary Incubation

None

Detection

By flow cytometry

Results Summary

Great staining for transcription factor. Make sure to permeabilize nucleus.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

Need careful titration

The Bottom Line

Great antibody specificity and bright fluorochrome.

Share your experience with other scientists. Write a Review! »

Join the discussion