IgG1 Control

Institute of Biomedical Engineering
University of Oxford
Ph.D. Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Purified Mouse IgG1, κ Isotype Ctrl Antibody

Catalog Number:

401401

This control provides an excellent standard when quantifying total IgG1 in murine serum samples.

Experimental Design and Results Summary

Applications

ELISA

Sample

Used as a standard to quantify total IgG1 in mouse serum

Primary Incubation

Overnight to bind to the plate

Blocking Agent

10% FBS in PBS

Secondary Incubation

2 hours in PBS

Tertiary Incubation

1 Hour with anti-IgG1 antibody conjugated to HRP

Detection

TMB Ultra substrate

Results Summary

This control provides an excellent standard when quantifying total IgG1 in murine serum samples

DOI or PMID #

For single sample extractions, care should be taken when aliquoting small volumes (e.g. 0.5 µL) to ensure accurate pipetting. Columns must be prepared for 10–20 mins prior to use.

Additional Notes

N/A

Image Gallery

Summary

The Good

Very useful as a standard and positive control

The Bad

N/A

The Bottom Line

This control provides an excellent standard and positive control

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