Working Dvl-3 Antibody with Moderate Specificity

Molecular Biology
Princeton
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

anti-Dvl-3 (G-7)

Catalog Number:

sc-271295

This antibody was used by conventional western blotting in multiple wnt-modulated cells. Results show 1 non-specific band but strong detection at the expected size.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human MDA-MB-231 breast cancer cells

Primary Incubation

1:1000 in 5% milk in IBS-T

Blocking Agent

5% milk in IBS-T

Secondary Incubation

1:10000 in 5% milk in IBS-T

Tertiary Incubation

N/A

Detection

Licor infrared

Results Summary

Strong band at the expected size was observed and levels were consistent with that seen of another Dvl-3 antibody from CST.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good signal at expected size

The Bad

Other strong, non-specific band

The Bottom Line

Works well, but better optimized antibodies are also likely available.

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