BacMam LAMP1 with RFP Tag as Lysosome Marker

Harvard Medical School
Cell Biology
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CellLight™ Lysosomes-RFP, BacMam 2.0

Catalog Number:

C10597

CellLight® Lysosomes-RFP, BacMam 2.0, provides an easy way to label lysosomes with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Experimental Design and Results Summary

Application

Live cell imaging

Starting Material

Cultured HeLa cells

Protocol Overview

We add 5 µl of the reagent to each well of the 6-well plate the night before we do the imaging.

Tips

Use the amount recommended by the manual. Need to optimize the time for optimal expression level and signal.

Results Summary

Nice RFP signal and correct localization showing the morphology of typical lysosomes.

DOI or PMID #

28475870

Additional Notes

N/A

Image Gallery

Summary

The Good

Convenient, efficient, and fast

The Bad

None

The Bottom Line

This reagent is very good as a lysosome marker for live cell imaging.

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