Mouse CD127 BV785

December 06, 2018

BlueskyReddit
Innate Immunity Department
German Rheumatism Research Center Berlin
Ph.D. Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 785 anti-mouse CD127 (IL-7Rα) Antibody

Catalog Number:

135037

I am interested in innate lymphoid cells of different organs and thus need antibodies to identify populations. This antibody gave bright staining on small intestinal lymphocytes of C57BL/6 mice.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse small intestinal lymphocytes

Primary Incubation

CD127 BV785, clone A7R34 15 min at 37°C in PBS/BSA

Blocking Agent

Anti FcgR, clone 2.4G

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS Symphony

Results Summary

This antibody reliably stains the IL-7 receptor on lymphocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Nice resolution for cytokine receptor

The Bad

Nothing bad to say

The Bottom Line

Nice and reliable staining.

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