Mouse CD49b PE

December 04, 2018

BlueskyReddit
Innate Immunity Department
German Rheumatism Research Center Berlin
Ph.D. Student

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Company:

Miltenyi Biotec

Product Name:

CD49b PE, mouse

Catalog Number:

130-102-337

I am interested in innate lymphoid cells of different organs and thus need antibodies to identify populations. Staining of CD49b on NK cell in C57BL/6 mouse lung was bright and bimodal.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lung lymphocytes

Primary Incubation

CD49b PE, clone DX5; 15 min at 37°C in PBS/BSA

Blocking Agent

Anti FcgR, clone 2.4G

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS Symphony

Results Summary

NKp46+ NK cells express CD49b (DX5).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright and bimodal staining

The Bad

Titer 1:30

The Bottom Line

Bright staining; NK cells can be identified.

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