Very Good Mouse CD3 Antibody

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-mouse CD3epsilon Antibody

Catalog Number:

100306

Our lab studies the function of T cells in an influenza model in mice. In order to select these T cells for further downstream analysis, we need a very good and reliable CD3 marker. We chose this antibody because it’s bright and stable and we are confident that it will work within all our samples.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse blood

Primary Incubation

Cells were stained in 100 µL FACS buffer with antibody at 1:100 for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100 µL FACS buffer.

Blocking Agent

FACS buffer

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSR II

Results Summary

This antibody works extremely well to stain mouse CD3 cells even when there is a lot of debris.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright and stable

The Bad

NONE

The Bottom Line

This is a great antibody for staining any mouse CD3+ cells.

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