Collagen IV Antibody for Immunofluorescence

December 12, 2018

Immunology
MSKCC
Research Fellow

Overall

Quality of Results

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Company:

Bio-Rad

Product Name:

Collagen IV Antibody

Catalog Number:

2150-1470

We are seeking to understand epithelial–lymphocyte interactions in healthy and transformed mammary gland. This antibody is excellent for staining the basement membrane.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PLP fixed mouse (C57BL/6) virgin mammary gland cryosections

Primary Incubation

1 in 400 for 3 hr at room temperature

Blocking Agent

1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% goat serum

Secondary Incubation

Goat anti-rabbit Alexa Fluor 647 (Thermofisher, A-21245) 1:500

Tertiary Incubation

N/A

Detection

N/A

Results Summary

20 um cryosections of murine virgin mammary gland were stained for E-Cadherin (white), collagen IV (green), and counterstained with DAPI (blue).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reliable & reproducible staining of aSMA in mammary gland

The Bad

A bit pricey

The Bottom Line

Excellent staining of basement membrane.

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