Nice CD7 Antibody for Natural Killer Cells

Innate Immunity
Deutsches Rheuma-Forschungszentrum (DRFZ)
Graduate Student

Overall

Quality of Results

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Company:

BD Biosciences

Product Name:

BV786 Mouse Anti-Human CD7

Catalog Number:

740964

Browse Similar Products: CD7 Flow Cytometry Antibodies

We are using a number of antibodies in a multiparameter flow cytometry panel for the phenotyping of NK cells. Our research is focused on a subpopulation of NK cells which is specifically found after infection with Cytomegalovirus and can be defined by the combinatorial expression pattern of a number of markers. This antibody gives a very bright staining of CD7 on NK cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs

Primary Incubation

1:25, 15 min, room temperature

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSR Fortessa II

Results Summary

PBMCs were stained in 50 µl for 15 min at room temperature, washed with 130 µl PBS/BSA and acquired on a BD LSR Fortessa II. Figure shows cells gated on Lymphocytes/Single Cells/Viable CD14- CD19- cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very bright staining

The Bad

N/A

The Bottom Line

Good antibody. Gives very bright staining of CD7 on NK cells.

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