Lamp-1 Expression in THP-1 Cells

Vet. pathobiology
University of Missouri
scientist

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Company:

Fisher Scientific

Product Name:

CD107a (LAMP-1) Antibody eFluor 660

Catalog Number:

501124294

Browse Similar Products: LAMP-1 Immunofluorescence Antibodies

Monocytic THP-1 cells were infected with intracellular pathogen Salmonella typhi. Cells were then stained and viewed using fluorescence microscopy. Phagolysosome marker Lamp-1 was evaluated and compared in infected and control cells. The antibody has easy performance and works well.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Cell line

Primary Incubation

30 mins

Blocking Agent

Normal Goat Serum in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fluorescence Microscopy

Results Summary

Phagocytosis was determined by counting cells containing at least one bacterial cell per 300 cells from each sample. Phagolysosome marker Lamp-1 was significantly increased in infected monocytic THP-1 cells compared to control cells.

DOI or PMID #

N/A

Additional Notes

Make sure to wash 3X

Related Categories

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Summary

The Good

Easy performance

The Bad

N/A

The Bottom Line

The antibody has easy performance and works well for immunofluorescence.

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