Qiagen
Mouse Inflammatory Cytokines Multi-Analyte ELISArray™ Kits
Qiagen, MEM‐004A
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We measured common pro‐inflammatory cytokine levels in the WTFUS and mutFUS ACM by ELISA. Among those, we identified TNFα as the most highly altered secreted factor in mutFUS ACM (3.8‐fold increase over WTFUS ACM). Further, we validated this result using a second ELISA containing a new set of sandwich antibodies, and found mutFUS ACM contains significantly more TNFα compared to WTFUS or Non‐transduced (NT) ACM. This kit works great and saves time.
ELISA
Conditioned media of mouse astrocytes
For inflammatory array, Mouse Inflammatory Cytokines Multi‐Analyte ELISArray was run per manufacturer instructions (Qiagen, MEM‐004A).
Incubation time needs to be optimized
TNFα is the most highly altered secreted factor in mutFUS ACM (3.8‐fold increase over WTFUS ACM)
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Saves time
None
Highly sensitive multi-analyte ELISA kit