Western Blot Detection of DAB2IP

November 20, 2018

Biochemistry and Molecular Biology
University of Arkansas for Medical Sciences
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-DAB2IP antibody

Catalog Number:

ab87811

This Abcam polyclonal antibody to DAB2IP was used for the western blot detection of mouse (MEF) and human (293T) DAB2IP. This antibody is very specific and no background was seen in whole-cell lysates.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Whole-cell lysate from MEF and 293T cell lines (20 µg)

Primary Incubation

1:2000 dilution. 16 hours at 4°C in 1% Milk PBSTween

Blocking Agent

5% milk

Secondary Incubation

Donkey Anti-Rabbit IgG 1:5000 in 1% milk PBSTween

Tertiary Incubation

N/A

Detection

ECL+

Results Summary

This DAB2IP specific antibody easily detected the DAB2IP protein at ~132 kDa. Clean blot with no non-specific proteins showing up.

DOI or PMID #

N/A

Additional Notes

Very good, clean antibody

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Summary

The Good

Very clean and specific.

The Bad

None

The Bottom Line

Use this antibody for detection of mouse and human DAB2IP.

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