PAR4 Antibody (A-10)

Pediatrics
Drexel University
Postdoctoral Associate

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Company:

Santa Cruz Biotechnology

Product Name:

PAR4 Antibody (A-10)

Catalog Number:

sc-1666

Browse Similar Products: PAR4 Western Blot Antibodies

PAR4 (prostate apoptosis response 4) is specifically expressed by cells entering apoptosis and is not induced during growth factor stimulation, oxidative stress, necrosis, or growth arrest. The PAR4 gene encodes a protein with a putative nuclear localization signal and a carboxy terminal leucine zipper. PAR4 (A-10) is recommended for detection of PAR4 of mouse, rat, and human origin by western blotting (dilution 1:500). This antibody worked very well for my experiments.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lung

Primary Incubation

PAR4 (1:500 dilution), Overnight at 4°C

Blocking Agent

Odyssey blocking buffer (1:1)

Secondary Incubation

Goat anti-Mouse IgG, LICOR (1:5000 dilution) at RT for 1 hr

Tertiary Incubation

N/A

Detection

Scanned on Odyssey® CLx (auto scan 700 & s800)

Results Summary

Results were very good. I have reused PAR4 antibody for 5 more membranes and all worked well.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Mouse monoclonal PAR4 antibody from Santa Cruz is very good for western blots

The Bad

N/A

The Bottom Line

This antibody worked very well for my experiment. I recommend it for western blots.

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