Miltenyi Biotec
CD56+ CD16+ NK Cell Isolation Kit, human
130-092-660
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We used this kit in experiments in which we were investigating Fc-mediated functions of NK cells in the context of HIV infection. For this we needed to isolate NK cells from human PBMCs. This kit was used and consistently resulted in a high-purity yield of human NK cells.
Cell isolation
Human PBMCs
Isolate human PBMCs from whole blood. Wash, count cells, incubate with the biotin-labeled antibody cocktail followed by labelling with beads. According to manufacturer's instructions, apply labeled cells to column, wash 3X, elute. In the second step, label the target cells with CD16 beads and repeat the separation process with column.
Titrate the beads to save some beads, usually the amount of beads/antibody can be decreased while still giving the same isolation efficiency
The recovered cells show high purity of CD56-expressing and CD16-expressing NK cells.
N/A
Straightforward process, consistent results
Expensive
Great reagent to use when you need to work with high-purity CD56+/CD16+ NK cells (the majority of NK cells in the blood).