p21 PE Antibody for Flow Cytometry

November 16, 2018

R & D
Aptose Biosciences
Senior Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

p21 Waf1/Cip1 (12D1) Rabbit mAb (PE Conjugate)

Catalog Number:

8865

We used the antibody p21 PE conjugate to study p21 intracellular expression in PBMCs and had good results in detection.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMC

Primary Incubation

1 hr RT

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow

Results Summary

The antibody p21 PE conjugate was used to detect intracellular p21 in PBMC after fix and permeabilization. We followed the CST protocol and were successful at detecting p21.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good quality antibody for flow

The Bad

None

The Bottom Line

This antibody was good for intracellular p21 detection by flow cytometry.

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