High Molecular Weight Loading Control for Western Blot

November 15, 2018

BlueskyReddit
LMP
University of Toronto
Graduate Student

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Quality of Results

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Company:

Cell Signaling Technology

Product Name:

Vinculin (E1E9V) XP® Rabbit mAb

Catalog Number:

13901

Choice of a loading control for immunoblots is critical when assessing phenotypic effects at protein levels. Here, we utilized an antibody against vinculin in HCT116 cell lysates in shRNA samples (shGFP vs. shRNA target samples).

Experimental Design and Results Summary

Applications

Western Blot

Sample

HCT116 WCL

Primary Incubation

1:3000, Overnight at 4°C

Blocking Agent

5% skimmed milk - 10 min room temperature

Secondary Incubation

1:10,000, anti-rabbit HRP conjugated antibody, 30 min at room temperature

Tertiary Incubation

N/A

Detection

ECL reagent – film.

Results Summary

Good antibody for western blot with low background. Approx. size 100 kDa.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Clean antibody to use as a loading control

The Bad

N/A

The Bottom Line

Good loading control for any western blot

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