Proteintech
RNF156 Antibody
11285-1-AP
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I am interested in studying the molecular mechanism of hedgehog signal transduction in vertebrate cells. I have performed a CRISPR/Cas9 genetic screen to uncover new regulators of hedgehog signaling pathway. One of the negative regulators identified in my screens was MGRN1 (RNF156). In this experiment, I had knocked out MGRN1 in NIH-3T3 cells using lentiCRISPR v2 strategy. The result is a pooled knockout cell line for MGRN1. MGRN1 antibody from Proteintech picked up a band at ~70 kDa. The predicted size is 59 kDa.
Western Blot
NIH-3T3 whole-cell extract
1:150 dilution in 1xTBS-T; overnight at 4°C for 16 hr
5% Milk in TBS-T
1:10,000 dilution of donkey anti-rabbit IgG+HRP; 1 hr at room temperature
None
ECL
RNF156 antibody from Proteintech picks up a band at ~70 kDa. The band is specific for RNF156 (MGRN1) because the band intensity is reduced in CRISPR knockout lines.
N/A
OK for Western Blotting
Picks up a bunch of non-specific bands
Good for Western Blotting