Okay Antibody to Detect IL-2 Expression Using Flow Cytometry

November 16, 2018

Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

FITC Rat Anti-Mouse IL-2

Catalog Number:

554427

As an immunology lab, we routinely analyze the ability of T cells to make IL-2 since it's the first cytokine T cells make. The amount a T cell makes is very important since this cytokine helps T cells grow in an autocrine fashion at first. We stimulate T cells and assess IL-2 secretion using flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine CD8 T cells (in vitro)

Primary Incubation

1:200 in 1x BD Perm Wash buffer

Blocking Agent

FC blocking during surface staining (10 µg/mL)

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD Celesta

Results Summary

We are able to detect IL-2 expression using flow cytometry in CD8 T cells upon stimulation. We identified cells that make robust to moderate amounts of IL-2 but some that make none.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy way to measure IL-2 expression on a per-cell basis rather than ELISA as whole population.

The Bad

Have to use a high dilution to get good staining but FITC antibodies are cheaper for the most part.

The Bottom Line

Good antibody to detect IL-2 expression using flow cytometry in T cells.

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