Great Multipurpose Imaging System for Many Applications

November 13, 2018

BlueskyReddit
Johns Hopkins School of Medicine
Pharmacology and Oncology
Graduate Student

Overall

Performance

Ease-of-Optimization

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Company:

UVP, LLC

Product Name:

Biospectrum Imaging System

Catalog Number:

81034601

As an immunology lab, we perform various molecular biology–based experiments where we routinely do PCR or western blot analysis. This imaging system allows us to easily view DNA gels and also use the instrument for chemiluminescence for immunoblotting analysis. The acquisition of the gels and blots using a computer makes it all digital rather than using film.

Experimental Design and Results Summary

Application

View DNA gels or chemiluminescence for western gels

Protocol Overview

Depending on whether it's being used for DNA or western blot adjust the stage. Select the correct preset settings within the application to adjust the lighting (UV for DNA and Chemi for western) and camera settings. With the camera setting, determine the length of the exposure. For DNA gels, we typically expose for 1–2 seconds. For western blotting, we do a minimum of 1 minute to a maximum of 60 minutes (for weak signals).

Tips

All depends on the reagents used for DNA. Western blotting is highly dependent on the reagents such as antibodies including secondary HRP and ECL substrate.

Features Summary

Easy-to-operate imaging system.

DOI or PMID #

29884702

Additional Notes

Takes a while to get used to the application.

Related Categories

Image Gallery

Summary

The Good

No longer need to use film for western blotting and all digital images. Never have to worry about over exposing. No longer need to scan.

The Bad

None

The Bottom Line

Great multipurpose imaging system for critical molecular biology applications. Western blotting is so much easier with a digital imager.

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