Great Antibody to Identify Naive/Memory T Cells Based on CD62L Expression

Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 605 anti-mouse CD62L Antibody

Catalog Number:

104438

Our lab studies CD8 T cell differentiation and function. T cell subsets within the spleen and in vitro are generally defined by CD44 and CD62L surface expression. To identify these subsets, I profile CD8 T cells for these markers to define either naive (CD62LHiCD44lo) and central memory (CD62LHiCD44Hi). This marker is really important to differentiate between these subsets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Splenocytes

Primary Incubation

1:1000

Blocking Agent

FC Block (10ug/ml)

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD Celesta

Results Summary

In a model of acute viral infection using adoptively transferred donor cells, I identified central memory cells within the donor population based on CD62L expression.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

1:1000 dilution makes the antibody go a long way. Bright antibody also!

The Bad

None really

The Bottom Line

Great antibody everyone uses only in this color since it's always reliable and fits nicely in the flow panel in this normally dim channel.

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