Easy-to-Use Method to Isolate Naive Human CD8 T Cells

Johns Hopkins School of Medicine
Pharmacology and Oncology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Stem Cell Technologies

Product Name:

EasySep™ Human Naïve CD8+ T Cell Isolation Kit

Catalog Number:

19258

We routinely purify naive human CD8 T cells for our T cell cultures. After obtaining the cells, we stimulate the T cells in various conditions to assess differentiation and function of the cells.

Experimental Design and Results Summary

Application

Cell purification

Starting Material

Human leukocytes from PBMCs

Protocol Overview

Follow instructions found in the kit as it varies depending on cell counts and such.

Tips

This product requires the EasyStep Magnet

Results Summary

Works really well. We obtain high yield viable purified human naive CD8 T cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good yield and viability. No column is needed.

The Bad

Kit is expensive and need to purchase a magnet for use.

The Bottom Line

Great reliable kit to obtain naive CD8 T cells.

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