Great Buffering Reagent

Johns Hopkins School of Medicine
Pharmacology and Oncology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Corning

Product Name:

HEPES

Catalog Number:

25-060-CI

Buffering the media is very critical to maintain normal pH levels for cultures. HEPES is an additional buffering system we use in conjunction with the standard carbonate buffering system.

Experimental Design and Results Summary

Application

Buffering Media

Starting Material

T cell media with all components

Protocol Overview

Make up the final media with all the components such as antibiotics, RPMI, and serum for 1 L. Add 10 mL of the 100x HEPES solution.

Tips

None

Results Summary

Buffering with HEPES keeps the media fresher for longer periods, and the cells are much happier and healthier.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy to use and sterile

The Bad

A bit pricey

The Bottom Line

A great reagent to help maintain the buffering of media especially with cultures that generate a lot of lactate such as T cells or tumor cells.

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