Quality Biological
ACK Lysing Buffer
118-156-721EA
Info: Browse Lysing Buffers
Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. We routinely conduct experiments with splenocytes that need removal of red blood cells (RBCs) before we can proceed with downstream applications. We use this buffer for that exact purpose of removing the RBCs.
T cell cultures
Murine spleens
Excise and process spleen to single-cell suspension. Pellet suspension then remove the sup. Use 2–3 mL of ACK buffer for about 2 minutes until RBCs are no longer present. Add 30 mL of PBS to bring back to isotonic concentration.
Make sure you bring back the tonicity with PBS.
We are able to remove all RBCs with simple ACK lysing.
N/A
Always reliable way to cheaply remove RBCs
None
Easy-to-use buffer to remove RBCs from spleen samples