Good Human C-Kit Antibody

November 01, 2018

R&D
Primity Bio
Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 605™ anti-human CD117 (c-kit) Antibody

Catalog Number:

313218

We were trying to subset stem cells/progenitor cells in human bone marrow, and one of the markers we decided to use is CD117. This antibody provides a decent signal with our human bone marrow mononuclear cell samples.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human bone marrow mononuclear cells

Primary Incubation

1:50 for 30 min at 4°C

Blocking Agent

Human FcX

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII

Results Summary

Good staining

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good staining

The Bad

None

The Bottom Line

Great antibody for human c-kit

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