Miltenyi Biotec
Propidium Iodide Solution
130-093-233
Info: Browse Propidium Iodide Products
Our lab routinely performs multicolor flow cytometry of immune cells. To help gate out dead cells, we have tried propidium iodide to stain dead cells. Compared to DAPI, propidium iodide does not give as distinct of a separation between live and dead cells.
Flow cytometry
Human PBMCs
Collect and wash cells, dilute propidium iodide solution to optimized concentration, stain with flow antibodies and diluted propidium iodide, wash cells, then acquire
Optimize propidium iodide dilution
Staining of dead cells with enough discrimination between live and dead to draw a gate; however, the separation is not as clean as is seen with DAPI.
N/A
Easy to perform live/dead staining
Live/dead distinction is not as good as DAPI
Use DAPI for live/dead discrimination