Anti-Mouse CD3 Antibody for Flow Cytometry

October 29, 2018

BlueskyReddit
Cell Biology and Molecular Genetics
University of Maryland, College Park
Post-doctoral researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

PerCP/Cy5.5 anti-mouse CD3 Antibody

Catalog Number:

100217

Our lab studies immune responses to infection in mouse models. The detection of various immune cell subsets is important in delineating the immune responses in various tissue compartments. The use of multicolor flow cytometry to detect these cell populations is one method we use to characterize immune responses.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse brain homogenate

Primary Incubation

20 minutes on ice

Blocking Agent

FcR Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Detection of CD3 cell populations isolated from murine tissue specimens

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Stains desired antigen when used at optimal dilution

The Bad

None

The Bottom Line

Great antibody for detecting murine T cells

Share your experience with other scientists. Write a Review! »

Join the discussion