Plasmid MaxiPrep Kit

University of Maryland, College Park
Cell Biology and Molecular Genetics
Post-doctoral researcher

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Company:

Invitrogen

Product Name:

PureLink™ HiPure Plasmid Filter Maxiprep Kit

Catalog Number:

K210016

One focus of the research in our lab is to study immune responses against viral infections. This involves transduction of T cells with vectors expressing various immune molecules. To prepare these constructs for transduction, we use reliable kits that provide clean plasmid extracts suitable for this application.

Experimental Design and Results Summary

Application

Plasmid extraction

Starting Material

Competent bacterial cells transformed with human DNA plasmids

Protocol Overview

Harvest bacterial cells containing plasmid, equilibrate column, lyse cells, load filters, wash, elute.

Tips

Prepare all regents ahead of time

Results Summary

Pure DNA with high yields, suitable for many different downstream applications

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

High plasmid yields with great purity

The Bad

Time consuming

The Bottom Line

Great kit for plasmid preps

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