Cell Signaling Technologies
Alexa Fluor® 488 Phalloidin #8878
8878S
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Our aim was to determine the expression of Setdb1 in mouse epithelial cells. Phalloidin was used to define the cells under fluorescence.
Immunofluorescence
Mouse epithelial cells
Overnight, 4 degree C, 1:100
2% normal goat serum+ 10% BSA in PBS
Goat anti rabbit
Phalloidin
Fluorescence
Protocol for phalloidin was followed according to manufacturer's instructions. The stain gave a nice color to the cytosolic region in contrast to Setdb1 expression detected in the nucleus.
N/A
Gives nice contrast and color to cell bodies.
None
It is a good product to use as a stain to show where the cell bodies are with respect to your protein of interest. It can replace the need to take images in the bright field.