Good Vinculin Antibody for Western Blot

October 08, 2018

BlueskyReddit
School of Graduate Studies
SUNY Downstate Medical Center
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Vinculin antibody [EPR8185]

Catalog Number:

ab129002

Our aim was to determine changes in methylation pattern of H3K9me3 by changing Setdb1 expression in PC3 cells. For this study, vinculin was used as one of the controls to determine total protein loaded per sample.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell lines PC3 lysates

Primary Incubation

Overnight, 4 degree C, 1:5000

Blocking Agent

5% milk

Secondary Incubation

Goat anti rabbit

Tertiary Incubation

N/A

Detection

Hrp-dab reaction

Results Summary

Changes in methylation pattern of H3K9me3 were not observed by changing the expression of Setdb1. Vinculin was used as a loading control.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Highly specific, no cross reactivity

The Bad

None

The Bottom Line

Good control antibody for western blots.

Share your experience with other scientists. Write a Review! »

Join the discussion