Antibody for Fluorescent Myelin Staining

Pathology
Albert Einstein College of Medicine
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Millipore Sigma

Product Name:

Anti-Myelin Basic Protein Mouse mAb (SMI-99)

Catalog Number:

NE1019

Our lab frequently compares the differences in myelin in mouse models of Multiple Sclerosis. We use this antibody for fluorescent detection of MBP in IHC-paraffin samples.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse Spinal Cord Sections

Primary Incubation

1:1000 dilution in 5% milk, overnight at 4 degrees celcius

Blocking Agent

5% normal goat serum in 5% non-fat milk

Secondary Incubation

Goat anti-mouse- Alexa 488 in 5% non-fat milk ans 1X TBS

Tertiary Incubation

None

Detection

Flourescence

Results Summary

We were able to clearly detect myelin in the spinal coeds of mice with this antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Small volume will give great results

The Bad

High background if a concentrated amount is used

The Bottom Line

Great MBP staining by IHC

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