Anti-MAG Antibody

October 05, 2018

Pathology
Albert Einstein College of Medicine
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Millipore Sigma

Product Name:

Anti-Myelin Associated Glycoprotein Antibody, clone 513

Catalog Number:

MAB1567

We used this anti-body as an alternative to anti-MBP (Myelin Basic Protein) to detect the presence of myelin in mouse brain by IHC.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse Brain sections

Primary Incubation

1:250 in 5% normal goat serum in 5% non-fat milk incubated overnight at 4oC

Blocking Agent

5% normal goat serum in 5% non-fat milk

Secondary Incubation

Biotinilated, affinity purified anti-mouse antibody incubated for 1 hour at room temperature

Tertiary Incubation

None

Detection

DAB

Results Summary

We were not able to detect the presence of myelin by DAB using this anti-body

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

None

The Bad

Did not work for IHC

The Bottom Line

This antibody did not work on mouse brain by IHC-paraffin

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