CD16/CD32 Rat anti-Mouse, Unlabeled for FACS Blocking

Pathology
Albert Einstein College of Medicine
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

CD16/CD32 Rat anti-Mouse

Catalog Number:

553142

We wanted to analyse the specific cell types in the deep cervical lymph nodes of mice by FACS. There fore we used this antibody to Fc Block Fc receptors before incubating with our specific tagged antibodies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Lymph Node

Primary Incubation

1:100 in 0.5% BSA and 1% PBS

Blocking Agent

0.5% BSA in 1% PBS

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flourescent

Results Summary

This product was able to block all Fc receptors and reduce all the background.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Blocks all Fc Receptors and works after expiration date

The Bad

Expensive

The Bottom Line

The is a great antibody for Fc Block. Consistent quality.

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