Invitrogen
Invitrogen™ Platinum™ PCR SuperMix
11306016
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We used this product to amplify DNA isolated from mouse tails for sequencing. In summary, great amplification with no issues for downstream sequencing.
PCR
DNA
This supermix contains a mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient. I used 60uL of Platinum PCR supermix with 10uM of each primer and 2UL of DNA. The PCR was run as follows95*C 5 min (95*C 30 sec, 58*C 40 sec,72*C 40 sec) x40, 72*C 2 min,4*C Hold
Centrifuge before use
Great amplification. No issues with downstream sequencing.
N/A
Very easy and efficient
None
This is the best PCR Supermix I have come across